![]() ![]() Hypo-assembly of RNP granule components in RBP loss-of-function models has been linked to neurodevelopmental, neurodegenerative, and neuropsychiatric disorders, while granule hyper-assembly and maturation into aggregates notoriously cause neurodegenerative diseases ( 2, 3). Cytoplasmic compartmentalization via biomolecular condensation is evolutionarily ancient and supports the maintenance of cellular function in a broad range of tissues however, diseases linked to RNP granule homeostasis are predominantly associated with the nervous system. In contrast, cytoplasmic granules are typically characterized by the presence of specific proteins. In the nucleus, granules are often scaffolded by long noncoding RNAs (lncRNAs), termed architectural RNAs (arcRNAs). Ribonucleoprotein (RNP) granules are membrane-less cellular compartments composed of RNA and RNA binding proteins (RBPs) that interact with each other through multivalent interactions and dynamically exchange with the surrounding cellular milieu ( 1). Our work reports an architectural RNA for a neuronal granule and provides a handle to interrogate functions of a condensate independently of those of its constituent proteins.Ĭells partition their content as a strategy to coordinate the function of biomolecules in space and time. mimi granules contain mRNAs and proteins involved in synaptic processes granule loss in mimi mutant flies impairs nervous system maturity and neuropeptide-mediated signaling and causes phenotypes of neurodegeneration. Neuronal ELAV-like proteins directly bind mimi and mediate granule assembly, while Staufen maintains condensate integrity. Here, we show in Drosophila that a previously uncharacterized long noncoding RNA, mimi, is required to scaffold large neuronal granules in the adult nervous system. Neuronal granules are thought to condense around particular proteins that dictate the identity and composition of each granule type. The dysregulation of RNA-protein condensation disturbs synaptic plasticity and neuron survival and has been widely associated with human neurological disease. RNA binding proteins and messenger RNAs (mRNAs) assemble into ribonucleoprotein granules that regulate mRNA trafficking, local translation, and turnover. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |